At least 200,000 people have protested against the Czech government in the capital, Prague.
Demonstrators are calling for Prime Minister Andrej Babis to resign over allegations of fraud.
It is alleged Mr Babis used European Union subsidies for his private business - something he denies.
The protest was held on the eve of the 30th anniversary of the Velvet Revolution, which ended communist rule in what was then Czechoslovakia.
Saturday's demonstration was held in Letna Park, which was also the site of the biggest rallies of November 1989.
Many people have been chanting "we are here" - an echo of the revolution's protest slogan three decades ago.
And some of the speakers were former dissidents who also addressed the 1989 rallies.
As well as being prime minister, Mr Babis is the billionaire founder of a business called Agrofert - a Czech conglomerate holding company based in Prague.
The group that organised the protest, called the Million Moments for Democracy Association, is demanding that Mr Babis either resign by the end of the year or sever ties with the business.
Speakers at the rally also called for Czech President Milos Zeman to stand down.